monoclonal mouse anti human factor bb  (Quidel)

Journal: Neural Regeneration Research

Article Title: Gamma-glutamyl transferase 5 overexpression in cerebrovascular endothelial cells improves brain pathology, cognition, and behavior in APP/PS1 mice

doi: 10.4103/NRR.NRR-D-23-01525

Figure Lengend Snippet: GGT5 overexpression in cerebrovascular endothelial cells suppresses the IκB/NF-κB signaling pathway. (A) Histogram of GGT5 expression levels in the mouse cortex, as determined by qPCR ( n = 3 per group). (B, C) Volcano plots showing differential gene expression based on mRNA-seq analysis in the mouse cortex in the APP/PS1 + Vector group vs . the WT + Vector group (B) and the APP/PS1 + GGT5 group vs . the APP/PS1 + Vector group (C). (D) Venn diagram showing that there were 277 differentially expressed genes in the mouse cortex in common between the WT + Vector group vs . the APP/PS1 + Vector group and the APP/PS1 + GGT5 group vs . the APP/PS1 + Vector group, as determined by mRNA-seq. (E) Pathway enrichment analysis of genes that were differentially expressed between the APP/PS1 + GGT5 group and the APP/PS1 + Vector group (the NF-κB pathway is highlighted). (F) Heatmap displaying the relative expression levels of differentially expressed genes involved in the NF-κB pathway. (G) Histogram of Nfkbia expression, as determined by qPCR, in the mouse cortex ( n = 3 per group). (H–J) Representative western blots (each row is an image from a different blot) (H) and quantitative analysis of IκBα (I) and p-NF-κB/NF-κB (J) expression in the mouse cortex ( n = 3–5 per group). Data are expressed as mean ± SEM. * P < 0.05 (one-way analysis of variance followed by Tukey’s post hoc test). Birc3 : Baculoviral IAP repeat containing 3; Ctx: cortex; Gadd45b : growth arrest and DNA damage inducible protein beta; GGT5: gamma-glutamyltransferase 5; IκBα: inhibitor-kappaBα; NFKbia : nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitorα; NF-κB: nuclear factor‐kappa B; p-NF-κB: phosphor nuclear factor‐kappa B; Ptgs2 : prostaglandin endoperoxide synthase 2; qPCR: real-time polymerase chain reaction; Relb : recombinant v-rel reticuloendotheliosis viral oncogene homolog B; TNF: tumor necrosis factor; WT: wide type.

Article Snippet: The primary antibodies used were as follows: anti-GGT5 (human, mouse, 1:500, ABclone, Wuhan, China, Cat# A14374, RRID: AB_2761241), anti-agrin (AGRN; human, mouse, 1:500, Santa Cruz Biotechnology, Santa Cruz, CA, USA, Cat# sc-374117, RRID: AB_10947251), anti-LRP1 (human, mouse, 1:5000, Abcam, Cambs, Cambridge, UK, Cat# ab92544, RRID: AB_2234877), anti-amyloid-β (D54D2) (mouse, 1:1000, Cell Signaling, Boston, MA, USA, Cat# 8243, RRID: AB_2797642), anti-amyloid-β (1–42) (D9A3A) (mouse, 1:1000, Cell Signaling, Cat# 14974, RRID: AB_2798671), anti-APP C-terminal fragment (CTF; mouse, 1:1000, Sigma, Munich, Bayern, Germany, Cat# A8717, RRID: AB_258409), anti-BACE1 (D10E5) (mouse, 1:1000, Cell Signaling, Cat# 5606, RRID: AB_1903900), anti-NF-κB p65 (F-6) (mouse, 1:200, Santa Cruz Biotechnology, Cat# sc8008, RRID: AB_628017), anti-phosphor-nuclear factor kappa B (p-NF-κB) p65 (Ser468) (mouse, 1:1000, Cell Signaling, Cat# 3039, RRID: AB_330579), anti-inhibitor-kappaBα (IκBα) (H-4) (mouse, 1:100, Santa Cruz Biotechnology, Cat# sc-1643, RRID: AB_627772), anti-neuronal pentraxin II (NPTX2) (mouse, 1:5000, Abcam, Cat# ab277523, RRID: AB_3075509), and anti-β-actin (human, mouse, 1:5000, Boster, Cat# BM0627, RRID: AB_2814866).

Techniques: Over Expression, Expressing, Plasmid Preparation, Western Blot, Real-time Polymerase Chain Reaction, Recombinant

Journal: Journal of orthopaedic surgery and research

Article Title: Ferrostatin-1 inhibits osteoclast differentiation and prevents osteoporosis by suppressing lipid peroxidation.

doi: 10.1186/s13018-025-05544-2

Figure Lengend Snippet: Fig. 7 Fer-1 inhibits osteoporosis of OVX mice. (A) Reconstruction of bone trabecular of distal femurs by Micro-CT of each group. Scale bar = 200 μm. (B-F) Quantitative micro-CT analysis of bone mineral density (BMD), bone volume/total volume ratio (BV/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N), and trabecular separation (Tb.Sp). (G) HE staining of tissue sections, followed by the quantitative analysis of Tb.N and Tb.Sp of HE stain ing. Scale bar = 200 μm. (H) Relative concentration of TRAP, RANKL, CTX-1 and PINP in serum of each group. (I) TRAP staining of tissue sections. Scale bar = 200 μm. (J) Quantitative analysis of osteoclast number per trabecular bone surface (N. OC/BS). Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns = not statistically significant

Article Snippet: The cell supernatant or serum was collected and centrifuged for 20 min at 1000×g at 2–8°C and then added to a 96-well plate covered by ELISA antibodies against TGF-β (Elabscience, USA, E-EL-M1191), SDF-1 (Elabscience, USA, E-EL-M3046), S1P (Meimian, China, MM44778M2), PDGF-BB (Elabscience, USA, E-EL-M0632), Trap-5b (Elabscience, USA, E-EL-M1116), RANKL (Elabscience, USA, E-EL-M0644), CTX-1 (Elabscience, USA, E-EL-M3023) and PINP (Elabscience, USA, E-ELM0233).

Techniques: Micro-CT, Staining, H&E Stain, Concentration Assay